MCQ on Chromatography

Chromatography | Chromatograph

Gas chromatography

Paper chromatography

Thin layer chromatography

क्रोमैटोग्राफी | Chromatography in Hindi

गैस क्रोमैटोग्राफी | Gas chromatography (GC) in Hindi

पेपर क्रोमैटोग्राफी | Paper chromatography in Hindi

थिन लेयर क्रोमैटोग्राफी | TLC in Hindi


1. In which chromatography stationary phase is more polar than mobile phase?

A. Ion exchange chromatography
B. Normal phase chromatography
C. Reversed chromatography
D. Size exclusion chromatography

Correct Answer

2. In which type of chromatography, the stationary phase is held in a narrow tube andthe mobile phase is forced through it under pressure?

A. Column chromatography
B. Planar chromatography
C. Liquid chromatography
D. Gas chromatography

Correct Answer

3. Which of the following guidelines are applicable to Analytical Method validation


Correct Answer

4. In thin layer chromatography, the stationary phase is made of _________ and the
mobile phase is made of _________

A. Solid, liquid
B. Liquid, liquid
C. Liquid, gas
D. Solid, gas

Correct Answer

5. Which of the following multicomponent analysis technique involves estimation of
components that show change in spectra in different pH medium

A. Simultaneous equation
B. Derivative spectroscopy
C. Q Absorbance ratio
D. Difference spectroscopy

Correct Answer

6. In which of the following type of paper chromatography does the mobile phase move
horizontally over a circular sheet of paper?

A. Ascending paper chromatography
B. Descending paper chromatography
C. Radial paper chromatography
D. Ascending – descending chromatography

Correct Answer

7. In size exclusion chromatography, solute molecules are separated based on _________

A. Molecular geometry and size
B. Molecular composition
C. Molecular phase
D. Molecular formula

Correct Answer

8. Ion exchange chromatography is based on?

A. Electrostatic attraction
B. Electrical mobility of ionic species
C. Partition chromatography
D. Adsorption chromatography

Correct Answer

9. Which of the following is an example of bulk property or general detector in HPLC

A. Fluorescence detector
B. Refractive index detector
C. Electrochemical detector
D. UV-Visible detector

Correct Answer

10. Which of the following is used as a carrier gas in gas chromatography

A. Carbon dioxide
B. Oxygen
C. Helium
D. Methane

Correct Answer

11. 1H nuclei located near electronegative atoms tend to be ______ relative to 1H nuclei

A. Shielded
B. De shielded
C. Resonanced
D. Split

Correct Answer

12. Signal splitting in NMR arises from?

A. Shielding effect
B. Spin-spin decoupling
C. Spin-spin coupling
D. Deshielding effecr

Correct Answer

13. The base peak in mass spectrum is?

A. The lowest mass peak
B. The peak corresponding to the parent ion
C. The highest mass peak
D. The peak set to 100% relative intensity

Correct Answer

14. Which one of the following is necessary for mass spectrometry to occur?

A. Loss of an electron
B. Change of alignment of a proton in a magnetic field
C. A molecular vibration
D. Excitation of an electron from the ground state to a higher energy state

15. Column efficiency is measured in terms of number of theoretical plates, which is:

A. Inversely related to square root of height equivalent to theoretical plates
B. Directly related to square root of height equivalent to theoretical plates
C. Directly related to height equivalent to theoretical plates
D. Inversely related to height equivalent to theoretical plates

Correct Answer

16. In reverse phase HPLC, there is a

A. Non-polar solvent/polar column
B. Polar solvent/Non-polar column
C. Polar solvent/Polar column
D. Non-polar solvent/Non-polar column

Correct Answer

17. In a chromatographic separation, which of the following is most appropriate for the
qualitative analysis of a substance?

A. Taking factor
B. Capacity factor
C. Retention time
D. Resolution

Correct Answer

18. The basis of the technique of chromatography for separating components of a
mixture is?

A. the differing movement of particles of different mass in an electrical field
B. the interaction of the components with a stationary and a mobile phases
C. the absorption of infrared radiation by the components.
D. the deflection of charged particles in a magnetic field.

Correct Answer

19. HPLC is an abbreviation for?

A. High Profit Liquid Chromatography
B. High Pressure Liquid Chromatography
C. Higher Performance Low Chromatography
D. Higher Profit Low Chromatography

Correct Answer

20. The base value for the following compound is

A. 214
B. 253
C. 217
D. 202

Correct Answer

21. In IR spectroscopy, the wavenumber for nitrile group is observed in the range of

A. 3500-3300 cm-1
B. 2200-2100 cm-1
C. 1740-1650 cm-1
D. 3000-2800 cm-1

Correct Answer

22. Which of the following techniques would be most useful to identify as well as
quantify the presence of a known impurity in a drug substance?


Correct Answer

23. The results for precision studies in Analytical Method Validation, are expressed in
terms of?

A. % Relative error
B. Correlation coefficient
C. % Relative standard deviation
D. Mean

Correct Answer

24. Which of the following is used as a spraying reagent in paper chromatography?

A. conc. HCl
B. NaCl solution
C. Ninhydrin solution
D. CuSO4 solution

Correct Answer

25. In mass spectrometry, fragmentation of ions is achieved through?

A. Ionization
B. Splitting
C. Solubilization
D. Coupling

Correct Answer

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